Human iPSC-Derived Neural Stem Cells

Human iPSC-derived neural stem cells from healthy donors

Human iPSC-derived neural stem cells from patient donors

axolGEM (genetically edited model) human iPSC-derived neural stem cells

Neural cell culture media and reagents

Characterization

Phase contrast

Axol iPSC-Derived Neural Stem Cells grown in Neural Expansion-XF Medium with the addition of EGF and FGF2.

Two hours after thawing.

Two days after thawing.

Six days after thawing.

Immunofluorescence

Transcriptome

Transfection

Transfection of cerebral cortical neural stem cells with ReadyFect

ReadyFect leads to 30-40% transfection efficiency. The image demonstrates transfection results using 3 µL of ReadyFect / 1 µg of a GFP-encoding vector (pVectOZ-GFP). GFP expression was evaluated two days post-transfection.

Neurite outgrowth

Live imaging of neurite outgrowth with IncuCyte and NeuroTrack^TM by Essen Bioscience Ltd.

Competitor product comparision

Time-course comparison: N2a mouse neuroblastoma cell line, primary mouse cortical neurons (CTX), competitor's iPSC-derived neurons & Axol's cortical NSC-derived neurons

Patch clamp

Whole cell patch clamp recordings by Ole Paulsen's Lab (University of Cambridge)

Electrophysiological characterization of Axol's iPSC-Derived Cerebral Cortical Neurons

Multi-electrode array (MEA)

MEA analysis in Axol iPSC-Derived Cerebral Cortical Neurons

Human iPSC-derived neurons may be effectively used for drug discovery. Our collaborators, Ikurou Suzuki and his team at Tohoku Institute of Technology in Japan used Alpha MED Scientific’s multi-electrod array (MEA) system to investigate the functional characteristics of human iPSC-derived neurons on their long-term spontaneous activity and drug responsiveness.

The results from this research, using Axol iPSC-Derived Cerebral Cortical Neurons and culture reagents, can be found in the Posters and Product Citations sections below.

Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015).

Odawara A, Katoh H, Matsuda N, et al., Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture. Scientific Reports (2016).

Application of Axol iPSC-Derived Cerebral Cortical Neurons on multi-electrode arrays for studying network electrophysiology by Ole Paulsen's Lab (University of Cambridge)

Spontaneous spiking activity in Axol iPSC-derived neuronal cultures

Multi-electrode array studies of Axol iPSC-Derived Cerebral Cortical Neurons by Axion Biosystems

An activity map from the AxIS Software suite showing positions of spike activity for each well of the MEA

iPSC-Derived Cerebral Cortical Neuron morphology on Axion MEAs

• Next-generation neurological disease models: isogenic tools for investigating frontotemporal dementia, Alzheimer's and Parkinson's diseases

• Functional phenotypic characterization of iPSC-neurons from Alzheimer’s disease patients carrying PS-1 mutation in drug screening and disease modeling

• Characterization and potential applications of human iPSC-derived neural stem cells

• Modelling neurological disease: in vitro gene editing and iPSC differentiation combine to create powerful new tools

• Induction of plasticity phenomena in human induced pluripotent stem cell-derived cortical neurons

• Functional maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture

• Pharmacological responses in cultured human iPSC-derived cortical neurons using multi-electrode array

• In vitro electrophysiological drug testing using human induced-pluripotent stem cells

• Implementation of high throughput screening on iPS cells

• Using iPSC-derived neural stem cells as a CNS model to study neuronal behaviour in development and neurodegernation

• Modelling Alzheimer's disease: Development of a scalable, high-throughput-compatible assay to detect tau aggregates using iPSC-derived cortical neurons maintained in a 3D-culture format

• Karikari T.K, Nagel D.A, Grainger A et al., Preparation of stable tau oligomers for cellular and biochemical studies Analytical Biochemistry (2018)

• Kahn O.I, Schätzle P, van de Willige D et al., APC2 controls dendrite development by promoting microtubule dynamics Nature Communications (2018)

• van Pel D.M, Harada K, Song D et al., Modelling glioma invasion using 3D bioprinting and scaffold-free 3D culture Journal of Cell Communication and Signaling (2018)

• Nizou G, Wicks R.T, Wicks E.E et al., Human cortex spheroid with a functional blood brain barrier for high-throughput neurotoxicity screening and disease modeling Scientific Reports (2018)

• Basuodan R, Basu A.P, Clowry G.J et al., Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo Journal of Anatomy - Wiley Online Library (2018)

• Matsuda N, Odawara A, Katoh H et al., Detection of synchronized burst firing in cultured human induced pluripotent stem cell-derived neurons using a 4-step method Biochemical and Biophysical Research Communications (2018)

• Synder C, Yu L, Ngo T et al., In vitro assessment of chemotherapy-induced neuronal toxicity Toxicology in Vitro (2017)

• Rao A.N, Patil A, Brodnik Z.D et al., Pharmacologically increasing microtubule acetylation corrects stress-exacerbated effects of organophosphates on neurons. Traffic - Wiley Online Library (2017)

• Cho S.J, Park M.H, Han C et al., VEGFR2 alteration in Alzheimer’s disease. Scientific Reports (2017)

• Zollo A, Allen Z, Rasmussen H et al., Sortilin-Related Receptor Expression in Human Neural Stem Cells Derived from Alzheimer’s Disease Patients Carrying the APOE Epsilon 4 Allele. Neural Plasticity (2017)

• Gunhanlar N, Shpak G, van der Kroeg M et al., A simplified protocol for differentiation of electrophysiologically mature neuronal networks from human induced pluripotent stem cells. Molecular Psychiatry (2017)

• Jones VC, Atkinson-Dell R, Verkhratsky A et al., Aberrant iPSC-derived human astrocytes in Alzheimer's disease. Cell Death and Disease (2017)

• Poulsen ET, Iannuzzi F, Rasmussen HF, et al., An aberrant phosphorylation of amyloid precursor protein tyrosine regulates its trafficking and the binding to the clathrin endocytic complex in neural stem cells of Alzheimer's disease patients. Journal of Frontiers in Molecular Neuroscience (2017)

• O'Rourke C, Lee-Reeves C, Drake RAL, et al., Adapting tissue-engineered in vitro CNS models for high-throughput study of neurodegeneration. Journal of Tissue Engineering (2017)

• Wevers NR, van Vught R, Wilschut KJ, et al., High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform. Scientific Reports (2016)

• Szabo A, Kovacs A, Riba J, et al., The endogenous hallucinogen and trace amine N,N-dimethyltryptamine (DMT) displays potent protective effects against hypoxia via sigma-1 receptor activation in human primary iPSC-derived cortical neurons and microglia-like immune cells. Frontiers in Neuroscience (2016)

• Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)

• Odawara A, Katoh H, Matsuda N, et al., Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture. Scientific Reports (2016)

• Medda X, Mertens L, Versweyveld S, et al., Development of a scalable, high-throughput-compatible assay to detect TAU aggregates using iPSC-derived cortical neurons maintained in a three-dimensional culture format. Journal of Biomolecular Screening (2016)

• Cacabelos D, Ayala V, Granado-Serrano AB, et al., Interplay between TDP-43 and docosahexaenoic acid-related processes in amyotrophic lateral sclerosis. Neurobiology of Disease (2016)

• Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015)

• Verheyen A, Diels A, Dijkmans J, et al., Using human iPSC-derived neurons to model TAU aggregation. PLoS One (2015)

• Lin L, Juan Y, Bjoern S, et al., In vitro differentiation of human neural progenitor cells into striatal GABAergic neurons. Stem Cells Translational Medicine (2015)

• Relevance of iPS cells in cell based screening and drug development

• Frontotemporal dementia and Parkinson's Disease cellular models: In vitro characterization of pathological phenotypes in gene-edited iPSC-derived neurons with MAPT and LRRK2 mutations

• Abnormal phosphorylation of amyloid precursor protein tyrosine residues alters the APP trafficking in neurons from Alzheimer's Disease affected patients

• Modeling FTDP-17 linked tauopathies and Alzheimer's disease with human iPSC

• Electrophysiological maturation and pharmacological responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture

• The use of iPSC-derived cells and primary cells as in vitro models for toxicity screening

• Modeling Alzheimer's disease using stem cells

• Rising to the challenges of human iPSC-derived cells for tox & drug screening

• Induced Pluripotent Stem Cells: recapitulating disease to facilitate drug discovery

• Using human iPSC-derived cortical neurons for the early prediction of neurotoxicity

• Are 3D neural cell cultures the best translational models towards finding a cure for Alzheimer's disease?

• Using neural stem cells for safety pharmacology studies

• A suffering relationship in Alzheimer's disease

• iPSC-Derived Neurons for Epilepsy Studies

• Nurturing Neuroscience