Stem Cell Reprogramming

A complete suite of services

Our custom reprogramming and characterization services empower you to focus on your research interests and not use up your valuable time and resources generating cells

Simply send us your donor cells (fibroblasts or peripheral blood mononuclear cells (PBMCs)) and we'll use cutting edge reprogramming technologies (non-integrating episomal or Sendai virus reprogramming) to produce induced pluripotent stem cells (iPSCs) within a short period of time (usually 2 to 3 months)
If you are struggling to find the right donor cells for reprogramming, we can also help you source fibroblasts and PBMCs with a wide range of donor backgrounds from our established network.
Once you receive the iPSCs from us, our dedicated specialists will share their expertise and provide ongoing support to help ease the adaptation of the cells into your experimental systems.

What we can do for you

Our reprogramming service includes:

✓ Recovery, passaging, initial testing and banking of donor fibroblasts or PBMCs
✓ Cell reprogramming with non-integrating, zero-footprint systems:
i. Episomal vectors or ii. CytoTune™-iPS 2.0 Sendai virus
✓ Analysis of pluripotency using immunocytochemistry and TaqMan hiPSC Scorecard Assay

Additional 'add-on' services include:

✓ Human pathogen testing
✓ Short-Tandem Repeat (STR) profiling
✓ qPCR analysis for pluripotency markers
✓ Karyotyping (G-Banding)
✓ Provision of more than one iPSC colony from a single donor
✓ Assesment of reprogramming vector clearance
✓ Checking expression of additional marker genes by immunocytochemistry
✓ Sequencing genomic regions of interest

Related services:

✓ Sourcing donor fibroblasts and PBMCs
✓ Adapting your iPSCs from a feeder layer to a feeder-free system
✓ iPS cell diffferentiation to multipe important cell lineages and supply of ready-to-use/cryopreserved cells
✓ Genome editing and creation of isogenic cell lines with CRISPR-Cas9

Timeline

Our workflow

Axol's Stem Cell Reprogramming Service Timeline

Let us know

Do you have any questions about our services?

Our dedicated Services team can help you. Our custom services can be tailored to meet your experimental requirements, helping you to save time and resources.

I've been able to grow all the cells that Axol generated for me from my patient lines. All the cells provided grew well and I'm very happy with the service as well as the quality of the cells.
Dr David Millar • Human Molecular Genetics, Cardiff University

Axol has provided us with more than a fast and reliable supply of functional cortical human iPSC derived neurons. It has also given us access to the collective expertise of Axol, whom we now view as much a collaborator as a company.

Dr Eric Hill • Clinical and Systems Neuroscience, Aston University

Axol Bioscience has been a valued and important supplier to our lab’s research since the company was founded. The cells supplied to us by Axol represent exceptional value for money, but, most importantly, save us a great deal of valuable research time. Throughout our dealings with the company, service and support have always been excellent.

Dr Paul Charlesworth • Physiology, University of Cambridge

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Takeaways

Resources

White Paper

How to choose the best cell reprogramming approach for your research needs

Induced Pluripotent Stem Cells: recapitulating disease to facilitate drug discovery

Blogs

Reprogramming somatic cells to induced pluripotent stem cells (iPSC): A cellular career change

Assessing the pluripotency and the differentiation potential of iPSCs

Layers of complexity: differentiating iPSCs into derivatives of the embryonic germ layers

iPSC-Derived Cells

Disease Models

Cell Lines

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Primary Cells

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